tag:blogger.com,1999:blog-3693238014525650573.post3778452836850889346..comments2024-03-19T00:20:07.007-07:00Comments on News in Proteomics Research: Playing with the OpenMS Proteome Discoverer community nodes!Unknownnoreply@blogger.comBlogger1125tag:blogger.com,1999:blog-3693238014525650573.post-87630740786538380892016-02-08T16:59:20.530-08:002016-02-08T16:59:20.530-08:00Hi Ben,
I am not exactly sure what this rave is a...Hi Ben,<br /><br />I am not exactly sure what this rave is all about recently released openMS quantitation modules? I manually checked peptide XIC values for 3 charge states of the peptide with very good intensity and they were all 2-3 times off the values calculated manually by XCalibur, while already implemented PD very own Ion Area Detector is only 3% off of XCalibur manually calculated values. The same situation was repeated for other signals. And if openMS can not get peptide levels right then what are the chances of correct protein Label Free quantitation ? And it is not like those modules has a lot of parameters to adjust. So the question is if PD Ion Area Calculator does the job fabulously well whats the point of introducing additional modules, which I am not that sure undergone rigorous testing. Anonymoushttps://www.blogger.com/profile/11374791847540413909noreply@blogger.com