tag:blogger.com,1999:blog-3693238014525650573.post1930772843898042000..comments2024-03-19T00:20:07.007-07:00Comments on News in Proteomics Research: N15 labeling in Proteome DiscovererUnknownnoreply@blogger.comBlogger4125tag:blogger.com,1999:blog-3693238014525650573.post-15659189394034206312015-06-14T04:26:52.474-07:002015-06-14T04:26:52.474-07:00Yeah, thats tricky. If it was all static I'd ...Yeah, thats tricky. If it was all static I'd search with a "super modification" that has the mass of the phospho+the mass of the N15 incorporation, would it work with a dynamic modification? I'm not sure....Benhttps://www.blogger.com/profile/18385704761413487532noreply@blogger.comtag:blogger.com,1999:blog-3693238014525650573.post-39279722396431703232015-06-09T07:56:03.720-07:002015-06-09T07:56:03.720-07:00Hi Ben,
Thank you for this useful information.
Now...Hi Ben,<br />Thank you for this useful information.<br />Now I'm doing plant N15 labeling phosphoproteomics analysis, and I'd like to use PD to search N15 phosphopeptide results. However, Sequest can't allow two mods/amino aicd as you mentioned for N15-labeled Ser/Thr/Tyr, so I'd like to know if you have any suggestions for using PD searching. Thank you so much.Chuan-Chih Hsunoreply@blogger.comtag:blogger.com,1999:blog-3693238014525650573.post-79818601334992956552014-05-06T19:10:53.731-07:002014-05-06T19:10:53.731-07:00This comment has been removed by the author.PolearBearhttps://www.blogger.com/profile/05498918404615339435noreply@blogger.comtag:blogger.com,1999:blog-3693238014525650573.post-43105058867180127782014-05-05T22:14:13.566-07:002014-05-05T22:14:13.566-07:00Hi Ben, nice blog! And I'm a fan of PD too!
Re...Hi Ben, nice blog! And I'm a fan of PD too!<br />Recently I'm trying 15N labelling to perform quantification in my experiment, this is exactly what I'm looking for.<br />The attachments of .pptx seem broken now, could you please upload a .pdf version? Thank you in advance!PolearBearhttps://www.blogger.com/profile/05498918404615339435noreply@blogger.com