tag:blogger.com,1999:blog-3693238014525650573.post8507851006835100600..comments2024-03-28T08:46:55.598-07:00Comments on News in Proteomics Research: Want to monitor global redox response? Try cysTMTRAQUnknownnoreply@blogger.comBlogger1125tag:blogger.com,1999:blog-3693238014525650573.post-66357153612583985082014-10-22T10:30:28.510-07:002014-10-22T10:30:28.510-07:00Hi Ben, congratulations for your "must-read&q...Hi Ben, congratulations for your "must-read" blog. For sure, redox proteomics is becoming a hot-topic nowadays, and researchers are developing new approaches to identify/quantify the redox state of the cell. Your comment at the end of the post is quite important: "When I review a paper and it says "drug treatment leads to a decrease in phosphorylation in this list of proteins" my first thought is how do we know protein abundance didn't shift?" And papers like this one helps to answer this question. But this is not something new... In 2012, Martinez-Acedo et al. published in MCP (http://www.mcponline.org/content/11/9/800.full) a method called GELSILOX for GEL-based Stable Isotope Labeling of OXidized Cys, a method that allows the simultaneous quantification of proteins and of reduced and oxidized Cys sites in the same experiment. To do that, they used commonly used alkylating agents such as iodoacetamide and N-ethylmaleimide (to discriminate between oxidized and reduced cysteines) and label the whole proteome with oxygen 18. And no double tag was used.... Hope you will also enjoy it.Anonymousnoreply@blogger.com