Wednesday, February 26, 2020

Moving beyond trypsin with a photocleavable reagent!!


Busy week here and this one definitely deserves more time than I can afford this morning.

Highlights?

-- 30 minute digestion

-- High enough efficiency to be competitive with our friend trypsin

-- Doesn't care that youre integral membrane proteins have no basic residues, so better coverage of membrane proteins than I think I've ever seen before, but -- again, I'm moving fast.


1 comment:

  1. I synthesized this after I came across their Nature methods paper last year (https://www.nature.com/articles/s41592-019-0391-1). Eager to use this on some lysosomal fraction lying around!

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