The ULTIMATE Proteome Crosslinking Protocol!

THIS IS THE BLYBERMONDAY/CRACK FRIDAY SPECIAL I WAS LOOKING FOR!

EVERY step of the process.

A bunch of stuff I hadn't even thought about, like cell lysis, reaction conditions, offline fractionation (including what fractions to keep and which to toss)

AND A PRIDE FTP SITE WITH PROOF THAT ALL THIS WORKS!!

Is it my data processing pipeline? Nope! Cause now I have files where Proteome Wide Crosslinking actually worked!!