what about this awesome new paper and completely new strategy for pulling down phosphotyrosine peptides?!?!? (Big shoutout to Saddiq for tipping me off to it!)
What is it? A completely different way to pull down peptides with phosphotyrosines on them. SH2 domains of proteins specifically bind to phosphotyrosines. This group figured out if they took a protein and modified the SH2 domains they'd end up with proteins that'll bind P-Tyr with super strength!
How's it work? Better than any enrichment I've ever seen!
Proof? Orbi Velos files are at Proteome Exchange () here!