Saturday, December 31, 2016
Peptides with phosphotyrosine fragment differently when isobaric tags are used!
Okay...this one is weird...and fascinating!
The paper is from Robert Everley et al., and is in this month's JPR here!
These authors go into great detail in describing the chemistry and why all this makes sense. It involves bond energy and stuff.
They prove it by studying 2,000 synthetic peptides (!?!?!) labeled/unlabeled and fragmented with CID HCD and ETD.
My question is -- now that we know more about how TMT phospho fragmentation works, can we use this info to better interrogate our data? Perhaps by weighting the search engines to expect a neutral loss when we are doing TMT-phospho studies?!!?