Tuesday, August 30, 2016
Top down proteomics on a CE-Orbitrap!
Lots going on in the world of capillary electrophoresis (CE) - electrospray these days! (Sometimes this is referred to as capillary Zone electrophoresis (CZE) as in this new paper from Yimeng Zhao et al.,
In this study, this group fractionates an intact yeast proteome via RPLC and takes their own CE source coupled to a QE HF and sees how many proteoforms they can get.
Their in-house CE system has some serious run time capabilities and it looks like they were doing 40 minute runs. They end up detecting at the MS1 level over 3,000 individual proteoforms and are able to get MS/MS confirmation on 580 of them.
You could argue that is a lot of run time for 580 proteins. That is 15 hours or so, but it shows the potential that optimized CE has for top-down proteomics!