Sunday, August 21, 2016
Proteomics and phosphoproteomics of Scott Syndrome!
Not the right Scott at all! And much more serious.
Scott Syndrome (I just learned this today) is an extremely rare bleeding disorder. How rare? Well, there is one guy in the UK who is confirmed to have it and is a registered blood donor. They call him ScottUK.
Some work has gone into studying the disease and conclusions have been drawn regarding how Ca2+ channels are involved, but more work needs to be done.
Enter Fiorella Solari et al., and an incredibly through proteomic analysis of platelets from unaffected people and ScottUK.
iTRAQ is used
Phosphoproteomics is used
HighPH reverse phase fractionation is used
A Q Exactive does the heavy lifting and
PD 1.4 plus a publicly available Excel Macro from Karl Mechteler's lab (??) for simplifying modified peptide output reports (find it under software tools!) is used to find the cool stuff
PRMs on an Orbitrap Fusion are used to confirm what they found.
The did this with activated and unactivated platelets AND they also use open searching techniques (with quantification) to study protein cleavage events.
Honestly, I'm unqualified to assess the biology on this, but if you have a woefully under-analyzed disease you wanted to know more about -- this is a great study to show you how to go from little information to tons of information about that disease!