Thursday, February 11, 2016
How should we store and handle all these peptides for best results?
This article, honestly, isn't the most exciting thing...but its real important. If we're going to get around to standardizing this field we're all going to have to start following the same protocols. In this study from Andrew Hoofnagle et et et et al., (40 or so people at different labs ALL over threw in on this!) these authors systematically assemble a list of "Recommendations for the Generation, Quantification, Storage and Handling of Peptides" for LC-MS.
How long is that peptide good for? Do we put those standards at 4C or -20 or -80? How many times can we freeze thaw before we chuck it? All super important things to keep in consideration and this might be the current definitive work on it.