Wednesday, September 23, 2015
Analysis of phosphopeptide enrichment strategies
About a year ago I had a great conversation with a scientist from Cell Signaling who described the work they were doing with differential phosphopeptide enrichment. Now they have some figures up that describe the awesome work they've been doing!
If you are still using the FACE technique or a series of different enrichment strategies leading up to FACE you might want to take a step back and think about what you want to get out of your samples. Is a generic anti-phosphotyrosine antibody still the best for what you want out of your analysis? If you are interested in pathways, for example, that preferentially use phospho-Ser, maybe there is a better option now than we had 5 years ago!