Wednesday, May 6, 2015
Nice 2D gel-based proteomics analysis of Parkinsons Disease
This is the first 2D gel paper I've read in a while. I like it for a lot of reasons. (One of them is the fact that I didn't have to do this gel...NOT something you want my help with...)
The paper in question is by Judy Triplett et. al., and is open access here. In this study they look at proteomics and phosphoproteomics of a PINK1 knockout mouse as a model of inherited Parkinson's Disease. Interesting model? Check!
As you can tell from the gel image above, the technique is solid. Whoever did this knows what they're doing. Differential proteomic analysis was done and SYPRO ruby was used for differential phosphoproteomics. Spot IDs were performed with an Orbi XL.
Changes that were observed were validated by western blots. All around, just a nice technical study. Its almost just a plus that this work provided some insights in the major pathways affected by familial Parkinson's disease.